Document Details
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Document Type |
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Article In Journal |
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Document Title |
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Synthesis and Characterization of Cefotaxime Conjugated Gold Nanoparticles and Their Use to Target Drug‐Resistant CTX‐M‐Producing Bacterial Pathogens Synthesis and Characterization of Cefotaxime Conjugated Gold Nanoparticles and Their Use to Target Drug‐Resistant CTX‐M‐Producing Bacterial Pathogens |
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Document Language |
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English |
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Abstract |
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Multidrug-resistance due to “b lactamases having the expanded spectrum” (ESBLs) in members of Enterobacteriaceae is a matter of continued
clinical concern. CTX-M is among the most common ESBLs in Enterobacteriaceae family. In the present study, a nanoformulation of cefotaxime
was prepared using gold nanoparticles to combat drug-resistance in ESBL producing strains. Here, two CTX-M-15 positive cefotaxime resistant
bacterial strains (i.e., one Escherichia coli and one Klebsiella pneumoniae strain) were used for testing the efficacy of “cefotaxime loaded goldnanoparticles.”
Bromelain was used for both reduction and capping in the process of synthesis of gold-nanoparticles. Thereafter, cefotaxime was
conjugated onto it with the help of activator 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide. For characterization of both unconjugated and
cefotaxime conjugated gold nanoparticles; UV-Visible spectroscopy, Scanning, and Transmission type Electron Microscopy methods
accompanied withDynamic Light Scattering were used.We used agar diffusion method plus microbroth-dilutionmethod for the estimation of the
antibacterial-activity and determination ofminimuminhibitory concentration or MIC values, respectively.MIC values of cefotaxime loaded gold
nanoparticles against E. coli and K. pneumoniae were obtained as 1.009 and 2.018 mg/L, respectively. These bacterial strains were completely
resistant to cefotaxime alone. These results reinforce the utility of conjugating an old unresponsive antibiotic with gold nanoparticles to restore its
efficacy against otherwise resistant bacterial pathogens. |
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ISSN |
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1097-4644 |
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Journal Name |
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Journal of Cellular Biochemistry |
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Volume |
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1 |
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Issue Number |
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1 |
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Publishing Year |
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1438 AH
2017 AD |
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Article Type |
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Article |
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Added Date |
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Sunday, May 28, 2017 |
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Researchers
| Shaikh Sibhghatulla | Sibhghatulla, Shaikh | Investigator | Doctorate | |
| Rizvi Syed Mohd | Syed Mohd, Rizvi | Researcher | Doctorate | |
| Shakil Shazi | Shazi, Shakil | Researcher | Doctorate | |
| Hussain Talib | Talib, Hussain | Researcher | Doctorate | |
| Alshammari Thamir M | Thamir M, Alshammari | Researcher | Doctorate | |
| Ahmad Waseem | Waseem, Ahmad | Researcher | Doctorate | |
| Tabrez Shams | Shams, Tabrez | Researcher | Doctorate | |
| Al‐Qahtani Mohammad H | Mohammad H, Al‐Qahtani | Researcher | Doctorate | |
| Abuzenadah Adel M | Adel M, Abuzenadah | Researcher | Doctorate | |
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